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| Environmental Technology Listing |
Title of Listing: | | BiMelyze(R) Mercury Immunoassay |
Category: | | Characterization Technologies |
Subcategory: | * | Soil, All Listings |
Media: | | Water, Sludge, Soil, Solid |
Contaminants: | | Mercury |
Web Site: | | |
Email: | | |
Technology Description: | |
BioNebraska Inc. has developed and commercialized the BiMelyze(R) Mercury Tube Immunoassay Kit and Soil Extraction Kit for easy, rapid and cost effective semi-quantitative field measurements. The immunoassay is being used commercially for site assessments and soil evaluations during environmental remediation projects. Significant cost savings have been documented by being able to have real time test results available to guide the work crews in the process of soil removal during remediation projects. This eliminates the need to wait for laboratory test results to determine whether the clean-up was sufficient or whether a return trip to the site is necessary to remove contaminated soil missed in the first attempt. Having real time results, provided by the immunoassay at the remediation site, also allows the project leader to minimize the occurrence of excavating and disposing as hazardous waste, soil that is actually below the action level. Typical application sites have included battery recycling plants, chemicals and dye manufacturing, gas transmission metering and compression stations, munitions and nuclear facilities.
The immunoassay is based on a monoclonal antibody specific to the mercuric ion. Dropper bottles are provided for easy sample extractions, dilutions, filtering and for reagent additions. Results may be obtained by visual comparison of the unknown sample to control differential photometer available separately from BioNebraska. Standard control soil samples at 5 ppm and 15 ppm, or at action levels are extracted and tested at the same time as the unknown test samples. The soil extraction time in ten minutes at ambient temperature is an acid mixture defined in the Kit instructions using acids supplied by the customer. The Tube Immunoassay can be run in approximately thirty minutes.
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Performance Status/
Limitations: | |
Status
The BiMelyze Mercury Immunoassay test method has been evaluated and published in the DOE Methods Compendium as Test Method MB-100. California EPA has a draft method that has been reviewed and is in the final process of certification. US EPA/OSW has reviewed the test method and is presently in the process of recommending the method to the Inorganic Work Group for inclusion in SW 846 as Method No. 4500.
Some customers have successfully used the BiMelyze immunoassay to analyze various solids, semi-solids and liquids such as paint, cinderblock and sludge. It appears that the method may also be limited in application to other matrices by the ability of the acid mixture to disassociate and oxidize mercury to the mercuric form.
A prototype dust wipe procedure has been successfully developed and evaluated in a mercury contaminated production facility. Several successful projects have used the BiMelyze Immunoassay with a prototype extraction procedure on mercury contaminated shell fish, fin fish, animal and bird tissue.
Limitations
Stability - The BiMelyze Mercury Tube Immunoassay Kit is stable for at least six months when stored at 4 degrees C. Test reagents, once reconstituted and diluted to proper strength for testing should be used within five days. Test samples should be neutralized and buffered using the supplied reagents to within a pH range of 6-8 for maximum sensitivity.
Interferences - High concentrations of chromium (>38,000 ppm) and silver (>80,000 ppm) in mercury contaminated soils show positive interferences. High concentrations of copper (>47,000 ppm) and gold (>1,400 ppm) show a negative interference. These high concentrations are not usually found in environmental soil samples. Our experience shows that the concentrations of these metals commonly found in soil have no effect on the mercury assay. It is not necessary nor advisable to add gold preservation solutions to any samples tested by this assay.
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Topics
Analytical Testing, Mercury, Technologies, Characterization
Additional Topics/Tags/Keywords
Immunoassay, Screening |
Organization: | | BioNebraska, Inc |
Address: | | 3820 NW 46th Street
| Phone: | Description | Number |
City: | | Lincoln | | Toll Free | (800) 786-2580 |
State/Province/Territory: | | NE | 2. | | |
Zip/Postal Code: | | 68524 | 3. | | |
Country: | | United States | 4. | Fax: | (402) 470-2345 |
Branch Locations: | |
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